The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 27, 2024
Filed:
Nov. 18, 2019
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Victoria Constandse, Cape Town, ZA;
Daniel Klass, Livermore, CA (US);
Alexander Lovejoy, Newark, CA (US);
Melissa Loyzer, Hamilton, CA;
Bronwen Miller, Cape Town, ZA;
Bernd Hinzmann, Berlin, DE;
Sophie Beckert, Neuzelle, DE;
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Signature Diagnostics GmbH, Potsdam, DE;
Abstract
Present disclosure provides a method including isolating DNA from a source, thereby providing a composition including the isolated DNA. The isolated DNA has at least first and second target regions, where the length of the second target region is greater than the length of the first target region. The method further includes quantifying a total mass of the isolated DNA, quantifying a first quantification cycle (C) of the first target region and a second Cof the second target region, and calculating a Q-ratio for the isolated DNA by dividing the second Cby the first C. The method further includes determining a value for a quality-mass constant (k), estimating a required input mass by dividing kby the Q-ratio, and preparing the isolated DNA for sequencing if the total mass of the isolated DNA in the composition is equal or greater than the required input mass.