The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 27, 2024

Filed:

Jul. 21, 2021
Applicant:

Dsm Ip Assets B.v., Heerlen, NL;

Assignee:

DSM IP ASSETS B.V., Heerlen, NL;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/02 (2006.01); A23L 27/30 (2016.01); C12P 19/12 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0073 (2013.01); A23L 27/36 (2016.08); C12P 19/12 (2013.01); C12Y 114/13079 (2013.01); A23V 2002/00 (2013.01);
Abstract

The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.


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