The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 20, 2024

Filed:

Aug. 06, 2018
Applicant:

Keck Graduate Institute, Claremont, CA (US);

Inventors:

Kiana Aran, Pasadena, CA (US);

Tara deBoer, Berkeley, CA (US);

Irina Conboy, Berkeley, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6825 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6811 (2018.01); C12N 9/22 (2006.01); G01N 27/327 (2006.01); G01N 27/414 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6825 (2013.01); C12N 9/22 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6811 (2013.01); G01N 27/3272 (2013.01); G01N 27/4145 (2013.01); G01N 27/4146 (2013.01);
Abstract

A digital biosensor for assaying a target nucleic acid and methods of using the biosensor for digitally detecting the target nucleic acid are disclosed wherein the biosensor includes a substrate having a substrate surface and at least two electrodes, a linker molecule having a first moiety conjugated to the substrate surface, a ribonucleoprotein (RNP) conjugated to a second moiety of the linker molecule, and a guide ribonucleic acid (gRNA) having a first sequence capable of binding to the inactive RNP and a second sequence capable of binding to the target nucleic acid.


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