The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 13, 2024
Filed:
Aug. 21, 2017
Huazhong Agricultural University, Hubei, CN;
Shuhong Zhao, Hubei, CN;
Shengsong Xie, Hubei, CN;
Changzhi Zhao, Hubei, CN;
Xinyun Li, Hubei, CN;
Xiangdong Liu, Hubei, CN;
Xiaosong Han, Hubei, CN;
Gaojuan Yang, Hubei, CN;
Yang Gao, Hubei, CN;
Yilong Chen, Hubei, CN;
Xiaoyong Du, Hubei, CN;
Yiliang Miao, Hubei, CN;
Yunlong Ma, Hubei, CN;
Xiaolei Liu, Hubei, CN;
HUAZHONG AGRICULTURAL UNIVERSITY, Hubei, CN;
Abstract
Provided is a pig genome-wide specific sgRNA library, a preparation method therefor, and an application thereof. The sgRNA is targeted at a pig genome-wide protein-coding gene, lincRNA and/or miRNA. Specifically, an sgRNA construct has the following structure: AL-N20-AR, wherein AL is the left homology arm sequence located at the upstream of the coding sequence of a pig specific SgRNA, N20 is the coding sequence of the pig specific SgRNA, and AR is a right homology arm sequence located at the downstream of the coding sequence of the pig specific sgRNA. The sgRNA library can be used for screening functional genes of a pig or for preparing a kit.