The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 13, 2024

Filed:

May. 19, 2018
Applicant:

10x Genomics, Inc., Pleasanton, CA (US);

Inventors:

Alexander Y. Wong, San Francisco, CA (US);

Jeffrey Mellen, Martinez, CA (US);

Kevin Wu, San Francisco, CA (US);

Paul Ryvkin, San Jose, CA (US);

Assignee:

10X GENOMICS, INC., Pleasanton, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/725 (2006.01); G16B 45/00 (2019.01); G16B 40/30 (2019.01); G16B 30/00 (2019.01); C12Q 1/686 (2018.01); G16B 30/10 (2019.01); C12Q 1/6881 (2018.01); C07K 16/28 (2006.01); C12Q 1/6883 (2018.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6881 (2013.01); C07K 14/7051 (2013.01); C07K 16/2809 (2013.01); G16B 30/00 (2019.02); G16B 40/30 (2019.02); G16B 45/00 (2019.02); C07K 2317/565 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6883 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01); G16B 30/10 (2019.02);
Abstract

Methods for screening clonotypes are provided. Data representing a plurality of cells from a single subject is obtained. The data represents a plurality of clonotypes. The data includes a plurality of contigs for each respective clonotype in the plurality of clonotypes. Each respective contig in the plurality of contigs comprises (i) an indication of chain type for the respective contig and (ii) a contig sequence of an mRNA of the respective cell. There is determined, using the data, for each respective clonotype in the plurality of clonotypes, a number of the plurality of cells that represent the respective clonotype. In some instances, more than one cell in the plurality of cells have the same clonotype in the plurality of clonotypes. In some instances, the plurality of clonotypes comprises 25 clonotypes and where the plurality of cells includes at least one cell for each clonotype in the plurality of clonotypes.


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