The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 13, 2024

Filed:

Aug. 13, 2021
Applicants:

Kyushu University, National University Corporation, Fukuoka, JP;

Konan Gakuen, Kobe, JP;

Nippon Suisan Kaisha, Ltd., Tokyo, JP;

Inventors:

Yuji Okita, Hachioji, JP;

Makoto Ito, Fukuoka, JP;

Rie Hamaguchi, Fukuoka, JP;

Hatsumi Goda, Fukuoka, JP;

Seiya Mochinaga, Fukuoka, JP;

Daisuke Honda, Kobe, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/6432 (2022.01); C12N 15/09 (2006.01); C12P 7/64 (2022.01); C12P 1/00 (2006.01); C12N 15/79 (2006.01); C12P 7/6427 (2022.01); C12P 7/6472 (2022.01); C12P 19/34 (2006.01); C12N 1/00 (2006.01); C12N 1/12 (2006.01); C12R 1/00 (2006.01); C12R 1/89 (2006.01); C10G 3/00 (2006.01); C12N 15/52 (2006.01);
U.S. Cl.
CPC ...
C12P 7/6432 (2022.01); C12N 15/09 (2013.01); C12N 15/79 (2013.01); C12P 1/00 (2013.01); C12P 7/64 (2013.01); C12P 7/6427 (2013.01); C12P 7/6472 (2013.01); C12P 19/34 (2013.01); C10G 3/00 (2013.01); C12N 1/00 (2013.01); C12N 1/125 (2021.05); C12N 15/52 (2013.01); C12R 2001/00 (2021.05); C12R 2001/89 (2021.05);
Abstract

A method for producing a microbial oil includes steps of: genetically modifying a labyrinthulid by disrupting and/or silencing a gene, or by transforming another gene in addition to the disruption and/or gene silencing of the gene, and culturing the labyrinthulid, such that a fatty acid composition accumulated in the labyrinthulid comprises an increased EPA content; and collecting the microbial oil having the increased EPA content from the labyrinthulid. The labyrinthulid before the modification is selected from (A) a labyrinthulid belonging to the genusor genusand having very weak or no activity of producing PUFAs via a PUFA-PKS pathway; and (B) a labyrinthulid belonging to the genusin which a host PUFA-PKS gene is disrupted or silenced to a very weak level. The increased EPA content is preferably not less than 11.5% of a total fatty acid composition.


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