The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 06, 2024
Filed:
Nov. 09, 2020
Applicant:
E.i. Spectra, Llc, Ketchum, ID (US);
Inventors:
Harold E. Ayliffe, Ketchum, ID (US);
Donald O'Neil, Ketchum, ID (US);
Assignee:
Orflo Technologies LLC, Ketchum, ID (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/686 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/6827 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6848 (2013.01);
Abstract
A method for analyzing a target nucleic acid includes diluting nucleic acid targets and filling pico to femto-liter sized wells such that they contain a single target nucleic acid and one or more amplification reagents, amplifying the target in the individual wells, distinguishing wells containing amplicon from the target and amplicon from a variant of the target generated by polymerase error by using two differently labeled-hybridization probes, one hybridizing to the target and one hybridizing to a specific variant of the target; and analyzing target amplicons.