The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 06, 2024
Filed:
Dec. 21, 2018
Applicant:
Max-delbrück-centrum Für Molekulare Medizin IN Der Helmholtz-gemeinschaft, Berlin, DE;
Inventors:
Ralf Kühn, Berlin, DE;
Eric Danner, Berlin, DE;
Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); A61K 31/7088 (2006.01); A61K 38/46 (2006.01); C12N 15/01 (2006.01); C12N 15/90 (2006.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); A61K 31/7088 (2013.01); A61K 38/465 (2013.01); C12N 15/11 (2013.01); C12N 15/907 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01);
Abstract
A method for modifying double stranded DNA (dsDNA) employing an RNA guided DNA endonuclease to generate two double strand breaks in the dsDNA molecule to be modified, and replacement of the sequence positioned between the double strand breaks with a substitute DNA sequence using the non-homologous end joining (NHEJ) pathway, and corresponding kits and compositions for modifying double stranded DNA molecules.