The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 06, 2024
Filed:
Oct. 02, 2019
Applicant:
Hoffmann-la Roche Inc., Little Falls, NJ (US);
Inventors:
Assignee:
Hoffmann-La Roche Inc., Little Falls, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0781 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0635 (2013.01); C12N 2500/30 (2013.01); C12N 2501/998 (2013.01); C12N 2501/999 (2013.01); C12N 2502/1114 (2013.01); C12N 2502/1157 (2013.01); C12N 2502/1185 (2013.01);
Abstract
Herein is reported a method for producing a thymocyte supernatant comprising the steps of co-cultivating thymocytes and mononuclear cells at a cell ratio of at least 0.5:1.2 in the presence of phorbol-12-myristate-13-acetate and Phytohemagglutinin M for up to 60 hours, and separating the co-cultivation medium from the cells and thereby producing the thymocyte supernatant.