The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 30, 2024
Filed:
Jun. 23, 2017
Carlsberg A/s, Copenhagen, DK;
Toni Wendt, Frederiksberg, DK;
Ole Olsen, Copenhagen S, DK;
Søren Knudsen, Malov, DK;
Hanne Cecille Thomsen, Valby, DK;
Birgitte Skadhauge, Birkerod, DK;
Magnus Wohlfahrt Rasmussen, Copenhagen V, DK;
Massimiliano Carciofi, Copenhagen, DK;
Alexander Striebeck, Frederiksberg C, DK;
Carlsberg A/S, Copenhagen V, DK;
Abstract
In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.