The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 23, 2024

Filed:

Dec. 23, 2019
Applicant:

Dh Technologies Development Pte. Ltd., Singapore, SG;

Inventors:

Subhakar N Dey, Lexington, MA (US);

Michal Weinstock, Framingham, MA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/00 (2006.01); G01N 33/74 (2006.01); G01N 1/34 (2006.01); G01N 1/40 (2006.01); G01N 30/04 (2006.01); G01N 30/72 (2006.01); G01N 30/02 (2006.01); G01N 30/06 (2006.01);
U.S. Cl.
CPC ...
G01N 33/743 (2013.01); G01N 1/34 (2013.01); G01N 1/4077 (2013.01); G01N 30/04 (2013.01); G01N 30/72 (2013.01); G01N 2001/4083 (2013.01); G01N 2001/4088 (2013.01); G01N 2030/027 (2013.01); G01N 2030/045 (2013.01); G01N 2030/067 (2013.01); G01N 2560/00 (2013.01);
Abstract

The teachings herein provide for a method of analyzing testosterone using mass spectrometry. The method entails combining in a vial or well, a tagging reagent that is reactive with testosterone, an aqueous precipitation agent that precipitate proteins from solution, and an internal standard solution, the internal standard solution containing a known concentration of an isotopically enriched testosterone and then adding to the vial or well, a sample containing or suspected to contain testosterone. The vial can then be mixed to cause simultaneous precipitation of proteins and reaction of any testosterone present with the tagging reagent to form a mixture of a precipitate and a liquid solution. The liquid solution can then be separated from any precipitate and then analyzed for testosterone using liquid chromatography tandem mass spectrometry.


Find Patent Forward Citations

Loading…