The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 16, 2024

Filed:

Nov. 27, 2019
Applicant:

Microsoft Technology Licensing, Llc, Redmond, WA (US);

Inventors:

Yuan-Jyue Chen, Seattle, WA (US);

Bichlien Nguyen, Seattle, WA (US);

Jake Smith, Seattle, WA (US);

Karin Strauss, Seattle, WA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); B01L 3/00 (2006.01); C12Q 1/6874 (2018.01); G06N 3/123 (2023.01); G11C 13/02 (2006.01); G16B 50/40 (2019.01);
U.S. Cl.
CPC ...
C12N 15/1093 (2013.01); B01L 3/502761 (2013.01); C12Q 1/6874 (2013.01); G06N 3/123 (2013.01); G11C 13/02 (2013.01); G16B 50/40 (2019.02); B01J 2219/00722 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/16 (2013.01);
Abstract

Electrically controlled hybridization is used to selectively assemble oligonucleotides on the surface of a microelectrode array. Controlled activation of individual electrodes in the microelectrode array attracts oligonucleotides in solution to specific regions of the array where they hybridize to other oligonucleotides anchored on the array. The oligonucleotides that hybridize may provide locations for subsequent oligonucleotides to hybridize. The active electrodes and the oligonucleotides in solution may be varied during each round of synthesis. This allows for multiple oligonucleotides each with different and specific sequences to be created in parallel. This is accomplished without the use of phosphoramidite chemical synthesis or template-independent DNA polymerase enzymatic synthesis. Oligonucleotides created with these techniques may be used to encode digital data. Fully assembled oligonucleotides may be separated from the array and sequenced, stored, or otherwise processed.


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