The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 16, 2024

Filed:

Sep. 21, 2020
Applicants:

Florida State University Research Foundation, Inc., Tallahassee, FL (US);

Gary K. Ostrander, Tallahassee, FL (US);

Inventors:

Teng Ma, Tallahassee, FL (US);

Ang-Chen Tsai, Tallahassee, FL (US);

Xuegang Yuan, Tallahassee, FL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 3/06 (2006.01); C12N 5/00 (2006.01); C12M 1/12 (2006.01);
U.S. Cl.
CPC ...
C12M 23/16 (2013.01); C12M 25/14 (2013.01); C12M 27/16 (2013.01); C12N 5/0062 (2013.01); C12N 2513/00 (2013.01); C12N 2527/00 (2013.01); C12N 2533/12 (2013.01); C12N 2533/14 (2013.01); C12N 2533/30 (2013.01);
Abstract

The subject invention concerns materials and methods for culture of cell aggregates. The subject invention utilizes three-dimensional (3-D) inserts comprising micro-channels having selected dimensions. The inserts are provided in or on tissue culture plates that can be supported on a programmable rocking platform/station, thereby providing for a hydrodynamic environment that promotes 3-D aggregation of cells cultured on the plates. The supporting rocker is programmed to provide motion that generates hydrodynamic conditions that support 3-D cell aggregation and long-term culture. The subject invention also concerns an apparatus comprising a tissue culture vessel that comprises a 3-D insert of the present invention, and a programmable rocking platform/station that can provide motion to the vessel provided thereon, thereby generating hydrodynamic conditions and wave motion that support 3-D cell aggregation and cell culture. The subject invention also concerns methods for growing 3-D aggregates of cells.


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