The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 09, 2024

Filed:

Feb. 06, 2016
Applicant:

Cell Idx, Inc., San Diego, CA (US);

Inventor:

David A. Schwartz, Encinitas, CA (US);

Assignee:

CELL IDX, INC., San Diego, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/00 (2006.01); G01N 33/535 (2006.01); G01N 33/574 (2006.01); G01N 33/533 (2006.01); C07K 16/44 (2006.01); C07K 16/00 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5743 (2013.01); C07K 16/00 (2013.01); C07K 16/44 (2013.01); G01N 33/533 (2013.01); G01N 33/535 (2013.01); G01N 33/54306 (2013.01); G01N 33/574 (2013.01); G01N 2474/20 (2021.08); G01N 2800/60 (2013.01);
Abstract

The present disclosure provides high-performance immunoreagents for use in a variety of immunologic assays and other related techniques. The immunoreagents comprise a primary antibody and a bridging antigen, wherein the bridging antigen is recognized by a detectable secondary antibody with high affinity. Also provided are compositions comprising panels of immunoreagents specific for multiple different target antigens and compositions comprising pairs of primary immunoreagents and their complementary detectable secondary antibodies. The paired primary immunoreagents and secondary antibodies are useful in a variety of immunologic assays, particularly in highly multiplexed assays, where the structure of the bridging antigen is varied in tandem with variation in the detectable secondary antibody, such that a multiplicity of immunoreagents are provided that are capable of simultaneously detecting a multiplicity of target antigens in a single assay. Also provided are kits comprising the immunoreagents, methods of immunologic assay using the immunoreagents of the disclosure, and methods of preparation of the immunoreagents.


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