The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 09, 2024
Filed:
Mar. 16, 2021
Applicant:
Fluent Biosciences Inc., Watertown, MA (US);
Inventor:
Sepehr Kiani, Watertown, MA (US);
Assignee:
Fluent Biosciences Inc., Watertown, MA (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6876 (2018.01); C12N 15/10 (2006.01); C12Q 1/686 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6876 (2013.01); C12N 15/1096 (2013.01); C12Q 1/686 (2013.01);
Abstract
This disclosure provides methods and systems for single-cell, multi-omic analysis of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile. Nucleic-acid-tagged antibody conjugates are used for simultaneous proteomic analysis along with the gene expression profiling.