The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 09, 2024
Filed:
Nov. 15, 2021
Applicant:
Envirologix Inc., Portland, ME (US);
Inventors:
Daniel Shaffer, Portland, ME (US);
Stephen A. Judice, Portland, ME (US);
Assignee:
ENVIROLOGIX INC., Portland, ME (US);
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/6853 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/689 (2018.01); C12Q 1/6851 (2018.01); C12Q 1/68 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6853 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/68 (2013.01); C12Q 1/689 (2013.01); C12Q 1/6851 (2013.01);
Abstract
Described and featured herein are isolated oligonucleotides containing, from 5′ to 3′, a nicking enzyme recognition sequence, at least 9 nucleotides that specifically bind a target nucleic acid molecule, and 2′ modified nucleotides. The isolated oligonucleotides may be used in compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a Nicking and Extension Amplification Reaction (NEAR) reaction.