The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 02, 2024

Filed:

Aug. 30, 2019
Applicant:

The Charles Stark Draper Laboratory, Inc., Cambridge, MA (US);

Inventors:

Vishal Tandon, Somerville, MA (US);

Charles A. Lissandrello, Newtonville, MA (US);

Jenna L. Balestrini, Boston, MA (US);

Jonathan R. Coppeta, Windham, NH (US);

Patricia A. Swierk, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/42 (2006.01); C12N 15/00 (2006.01); C12M 3/06 (2006.01); B01L 3/00 (2006.01); C12N 15/87 (2006.01);
U.S. Cl.
CPC ...
C12M 23/16 (2013.01); C12M 35/02 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 2300/047 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0887 (2013.01); B01L 2400/0496 (2013.01); C12N 15/87 (2013.01);
Abstract

Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.


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