The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 19, 2023
Filed:
Aug. 01, 2022
Case Western Reserve University, Cleveland, OH (US);
University Hospitals Cleveland Medical Center, Cleveland, OH (US);
Michael W. Jenkins, Cleveland, OH (US);
Yehe Liu, Cleveland, OH (US);
CASE WESTERN RESERVE UNIVERSITY, Cleveland, OH (US);
NATIONAL INSTITUTES OF HEALTH (NIH), Bethesda, MD (US);
U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), Bethesda, MD (US);
U.S. GOVERNMENT NIH DIVISION OF EXTRAMURAL INVENTIONS AND TECHNOLOGY RESOURCES (DEITR), Bethesda, MD (US);
Abstract
The present disclosure relates to a simple, fast, and low cost method for 3D microvascular imaging, termed 'scatter labeled imaging of microvasculature in excised tissue' (SLIME). The method can include perfusing a contrast agent through vasculature of a tissue sample. The contrast agent can include colloids and a dispersant. After the contrast agent is perfused through the vasculature, the vasculature of the tissue sample can be treated with a molecule that cross links with at least a portion of the dispersant to form a sticky, non-Newtonian polymer that prevents leakage of the contrast agent out of the vasculature of the tissue sample. The tissue sample can then be immersed in a solution comprising a clearing agent and subsequently imaged.