The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 19, 2023
Filed:
Mar. 26, 2018
President and Fellows of Harvard College, Cambridge, MA (US);
The General Hospital Corporation, Boston, MA (US);
David Benson Chou, Boston, MA (US);
Liliana S. Teixeira Moreira Leijten, Maastricht, NL;
Arianna Rech, Leicestershire, GB;
Richard Novak, Jamaica Plain, MA (US);
Donald E. Ingber, Boston, MA (US);
Yuka Milton, Cambridge, MA (US);
Viktoras Frismantas, Roxburg Crossing, MA (US);
Oren Levy, Brookline, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
The General Hospital Corporation, Boston, MA (US);
Abstract
The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.