Methods and apparatuses for predicting and using one or more resonance frequencies for efficient operation of an acoustofluidic cavity

Abstract

A method of predicting one or more resonance frequencies for efficient operation of an acoustofluidic cavity in an acoustofluidic device is provided. The method comprises the steps of i. providing a first liquid filling the cavity, ii. obtaining a first impedance spectrum, by measuring the electrical impedance of an ultrasound transducer connected to the acoustofluidic device as the transducer is driven at a range of frequencies, iii. providing a second liquid filling the cavity, wherein the second liquid differs from the first liquid in its acoustophysical properties, iv. obtaining a second impedance spectrum, by measuring the electrical impedance of the ultrasound transducer as the ultrasound transducer is driven at the range of frequencies, and v. predicting the one or more resonance frequencies for efficient operation of the acoustofluidic cavity by comparing the first and second impedance spectrum and selecting the one or more frequencies where the absolute value of the difference between the spectra, normalized by the absolute value of one of them, is largest. A method of driving an acoustofluidic device and apparatuses for predicting resonance frequencies and performing acoustofluidic operations are also provided.