The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 12, 2023
Filed:
Jun. 28, 2021
Deutsches Rheuma-forschungszentrum Berlin, Berlin, DE;
A·p·e Angewandte Physik & Elektronik Gmbh, Berlin, DE;
Toralf Kaiser, Birkenwerder, DE;
Daniel Kage, Falkensee, DE;
Claudia Giesecke-Thiel, Berlin, DE;
Konrad von Volkmann, Berlin, DE;
Deutsches Rheuma-Forschungszentrum Berlin, Berlin, DE;
A.P.E Angewandte Physik & Elektronik GmbH, Berlin, DE;
Abstract
The invention relates to a method and system for characterizing particles using a flow cytometer comprising detecting radiated light from the particles using two or more detectors positioned to allow for the detection in two or more angular directions and generating a waveform, as a digital representation for the detected radiated light for each of said angulation direction. The waveforms are transformed using one or more basis functions to obtain one or more coefficients characterizing the waveform. The one or more coefficients characterizing the waveform preferably correspond to properties of the particle(s), thereby enabling analysis of physical properties of the particles (such as size, shape, refractive index) or biological properties of the particles (such as cell type, cell cycle state or localization or distribution of molecules within the cell and/or on the cell surface). In preferred embodiments the method and system are used for a label-free sorting of particles, in particular biological cells.