The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 05, 2023
Filed:
Apr. 19, 2017
Global Life Sciences Solutions Usa Llc, Marlborough, MA (US);
John Richard Nelson, Clifton park, NY (US);
Robert Scott Duthie, Schenectady, NY (US);
Patrick McCoy Spooner, Slingerlands, NY (US);
John Anthony Schiel, Westminster, CO (US);
Lisa Anne Lowery, Niskayuna, NY (US);
Anja Josifa Smith, Thornton, CO (US);
GLOBAL LIFE SCIENCES SOLUTIONS USA LLC, Marlborough, MA (US);
Abstract
A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5' flanking sequence and a donor 3′ flanking sequence. The donor 5′ flanking sequence and the donor 3′ flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.