The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 28, 2023

Filed:

Jun. 07, 2017
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Daniel Takashi Kamei, Monterey Park, CA (US);

Benjamin Ming Wu, San Marino, CA (US);

Daniel William Bradbury, Los Angeles, CA (US);

Shin Ting Sherine Frieda Cheung, Los Angeles, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/543 (2006.01); C12Q 1/6844 (2018.01); C12Q 1/6813 (2018.01);
U.S. Cl.
CPC ...
G01N 33/54388 (2021.08); C12Q 1/6813 (2013.01); C12Q 1/6844 (2013.01); G01N 33/543 (2013.01); G01N 33/54306 (2013.01);
Abstract

In various embodiments methods and devices are provided for the detection and/or quantification of an analyte. In certain embodiments a device is provided comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase where, in use, said first phase solution becomes a leading phase and said second phase solution becomes a lagging phase; a lateral-flow assay (LFA); and a probe and/or a development reagent, where in use, said probe associates with said first phase solution in said leading phase of said ATPS and/or said development reagent associates with said second phase solution in said lagging phase of said ATPS. In certain embodiments a 'one-pot' system of purifying and amplifying a nucleic acid is provided utilizing, e.g., an ATPS and isothermal amplification reagents.


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