The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 28, 2023

Filed:

Dec. 31, 2020
Applicant:

Ampliwise Inc., Santa Clara, CA (US);

Inventors:

Kai Wu, Mountain View, CA (US);

Mindy Su, Cupertino, CA (US);

Xing Su, Cupertino, CA (US);

Assignee:

AMPLIWISE INC., Santa Clara, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6853 (2018.01); C12P 19/34 (2006.01); C12Q 1/6827 (2018.01); C12Q 1/686 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6853 (2013.01); C12P 19/34 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6827 (2013.01);
Abstract

An embodiment relates to system comprising: (i) a reaction mixture comprises: a) a target nucleic acid molecule; b) a forward primer complementary to a strand of the target nucleic acid molecule, the forward primer comprises a first molecular moiety at a 3' end, wherein the first molecular moiety is non-complementary to the strand of the target nucleic acid molecule; c) a reverse primer complementary to a complementary sequence of the strand of the target nucleic acid molecule, the reverse primer comprises a second molecular moiety at a 3′ end, wherein the second molecular moiety is non-complementary to the complementary sequence of the strand of the target nucleic acid molecule; d) a polymerase with 3′-5′ exonuclease activity; and (ii a device suitable of detecting an amplification products.


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