The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 28, 2023

Filed:

Dec. 10, 2020
Applicants:

Asymchem Laboratories (Tianjin) Co., Ltd., Tianjin, CN;

Asymchem Life Science (Tianjin) Co., Ltd., Tianjin, CN;

Tianjin Asymchem Pharmaceuticals Co., Ltd., Tianjin, CN;

Asymchem Laboratories (Fuxin) Co., Ltd., Liaoning, CN;

Jilin Asymchem Laboratories Co., Ltd., Jilin, CN;

Inventors:

Hao Hong, Tianjin, CN;

Gage James, Tianjin, CN;

Jiangping Lu, Tianjin, CN;

Na Zhang, Tianjin, CN;

Wenyan Yu, Tianjin, CN;

Fang Liu, Tianjin, CN;

Yanjun Li, Tianjin, CN;

Xin Huang, Tianjin, CN;

Juan Gao, Tianjin, CN;

Kejian Zhang, Tianjin, CN;

Yulei Ma, Tianjin, CN;

Junlu Wei, Tianjin, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/02 (2006.01); C12N 15/70 (2006.01); C12N 15/81 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0071 (2013.01); C12N 15/70 (2013.01); C12N 15/81 (2013.01); C12Y 114/11002 (2013.01);
Abstract

Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids; wherein the mutation of one or more amino acids must comprises E27K, and the mutation of one or more amino acids selected from the group consisting of: H14R, L16N, T25R, F26L, E27K, D30S, S33N, E34N, E34G, E34L, E34S, E34D, Y35W, Y35K, S37W, S37F, S37E, S37N, S37T, S37C, W40F, K41E, D54G, H55Q, S57L, I58T, I58Y, I58A, I58R, I58V, I58S, I58C, K86P, T91A, F95Y, C97Y, I98V, K106V, K106T, K106Q, F111S, K112E, K112R, S154A, K162E, L166M, I118F, I118V, I118R, H119R, H119F, I120V, K123D, K123N, K123Q, K123S, K123I, K123T, T130N, D134G, V135K, N165H, D173G, K209R, I223V and S225A, and having proline hydroxylase activity.


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