The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 21, 2023
Filed:
Aug. 21, 2020
Isoplexis Corporation, Branford, CT (US);
Luisa Andruzzi, Concord, MA (US);
Jimmy Adediran, Waltham, MA (US);
Timothy Pelletier, Leominster, MA (US);
Austin Ricker, Waltham, MA (US);
Angela DeLucia, Cambridge, MA (US);
Ben McNally, Medford, MA (US);
Dona Hevroni, Lexington, MA (US);
Mong Sano Marma, Natick, MA (US);
John Andrew Sheridan, Marblehead, MA (US);
IsoPlexis Corporation, Branford, CT (US);
Abstract
The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3'-O position capped by a disulfide-based 3′-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.