The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 21, 2023

Filed:

Jan. 10, 2018
Applicant:

Juno Therapeutics, Inc., Seattle, WA (US);

Inventors:

Mark L. Bonyhadi, Sammamish, WA (US);

David G. Kugler, Seattle, WA (US);

Timothy G. Johnstone, Seattle, WA (US);

Ronald James Hause, Jr., Seattle, WA (US);

Lucas J. Thompson, Seattle, WA (US);

Ryan P. Larson, Seattle, WA (US);

Assignee:

Juno Therapeutics, Inc., Seattle, WA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6827 (2018.01); G16B 30/10 (2019.01); C12Q 1/6806 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6886 (2018.01); G16B 20/20 (2019.01); G16B 40/10 (2019.01); G16B 20/10 (2019.01);
U.S. Cl.
CPC ...
C12Q 1/6827 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6886 (2013.01); G16B 20/10 (2019.02); G16B 20/20 (2019.02); G16B 30/10 (2019.02); G16B 40/10 (2019.02); C12Q 2600/142 (2013.01); C12Q 2600/154 (2013.01); C12Q 2600/156 (2013.01);
Abstract

Provided herein are methods of identifying genomic region(s) predictive of an outcome of treatment with a cell therapy and/or of a phenotype of function of the cells. In some embodiments, the methods include epigenetic and/or epigenomic analyses of the cells in connection with methods for preparing engineered cells for cell therapy and/or predicting response to a cell therapy, e.g., engineered cells for cell therapy. In some embodiments, the methods include steps to assess, characterize and analyze changes or modifications in an epigenetic property of gene region or regions, such as chromatin accessibility, nucleosome occupancy, histone modification, spatial chromosomal conformation, transcription factor occupancy and/or DNA methylation. In some embodiments, the epigenetic and/or epigenomic analysis includes determining the epigenetic properties of a cell, e.g., an engineered cell for cell therapy.


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