The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 21, 2023

Filed:

Mar. 30, 2020
Applicant:

Brandeis University, Waltham, MA (US);

Inventors:

Seth Fraden, Newton, MA (US);

Galder Cristobal-Azkarate, Bordeaux, FR;

Assignee:

Brandeis University, Waltham, MA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/38 (2006.01); B01L 3/00 (2006.01); C30B 7/00 (2006.01); C30B 29/58 (2006.01); B01L 3/06 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502784 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502746 (2013.01); C30B 7/00 (2013.01); C30B 29/58 (2013.01); G01N 1/38 (2013.01); B01L 3/06 (2013.01); B01L 3/5027 (2013.01); B01L 7/54 (2013.01); B01L 2200/0621 (2013.01); B01L 2200/0642 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/1822 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0655 (2013.01); G01N 2001/386 (2013.01); Y10T 137/0318 (2015.04); Y10T 436/2575 (2015.01);
Abstract

Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.


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