The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 14, 2023
Filed:
Oct. 29, 2020
Reprobiogen Inc., Toronto, CA;
Svetlana Madjunkova, Toronto, CA;
Clifford L. Librach, Toronto, CA;
REPROBIOGEN INC., Toronto, CA;
Abstract
A method is described for determining carrier status of an embryo for a balanced chromosomal rearrangement (BCR) prior to implantation of the embryo. The method involves obtaining cells from the embryo from at least day 4 post in vitro fertilization; conducting long-read nanopore sequencing of the DNA of the cells and the BCR carrier parent to detect at least one breakpoint; preparing primers specific to the breakpoint; employing the primers in a polymerase chain reaction customized to the breakpoint (cBP-PCR) to determine whether the breakpoint is indicative of BCR; and determining on the basis of cBP-PCR whether the embryo status is BCR carrier or BCR noncarrier; and conducting Sanger sequencing to determine balance in the embryo. The method can assist BCR carrier parents to pursue reproductive technologies while preventing vertical transmission of a BCR to offspring.