The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 14, 2023

Filed:

Aug. 29, 2019
Applicant:

Robert Bosch Gmbh, Stuttgart, DE;

Inventors:

Nadezda Fomina, Redwood City, CA (US);

Christopher Johnson, San Carlos, CA (US);

Young Shik Shin, Mountain View, CA (US);

Christoph Lang, Sunnyvale, CA (US);

Assignee:

Robert Bosch GmbH, Stuttgart, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 27/30 (2006.01); G01N 27/327 (2006.01); G01N 33/52 (2006.01); C12Q 1/6869 (2018.01); B01L 3/00 (2006.01); B01J 19/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); B01J 19/0093 (2013.01); B01L 3/502715 (2013.01); G01N 27/307 (2013.01); G01N 27/3272 (2013.01); G01N 27/3275 (2013.01); G01N 33/526 (2013.01); B01L 2300/0645 (2013.01);
Abstract

The present disclosure relates to systems, devices, and methods for nucleic acid sequencing including polynucleotide strands having a nucleotide(s) modified with a redox label(s) attached thereto or capable of receiving the modified nucleotide(s) with a redox label(s) attached thereto. The systems, devices, and methods include a dielectric member with an attached translocating protein positioned between oxidizing and reducing electrodes. The oxidizing and reducing electrodes generate an electrical field extending to a reaction area where the translocation of the polynucleotide strand through the protein occurs such the modified nucleotide(s) with redox label(s) attached thereto are identified by changes in current flow in the oxidizing and reducing electrodes, wherein the changes identify electron transfer from the reducing electrode, to redox label, and to oxidizing electrode when the modified nucleotide with a redox label covalently bonded to the nucleoside base of the modified nucleotide of the polynucleotide strand is at the reaction area.


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