The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 14, 2023

Filed:

Oct. 29, 2020
Applicant:

Progeneer, Inc., Seoul, KR;

Inventors:

So Yeon Ahn, Seoul, KR;

Srivithya Vellampatti Krishnamoorthy, Cheon Cheong-dong, KR;

Soong Ho Um, Seoul, KR;

Sung Oh, Seoul, KR;

Seung Won Shin, Gyeonggi-do, KR;

Assignee:

Progeneer, Inc., Seoul, KR;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 21/00 (2006.01); C07K 14/005 (2006.01); C07K 14/62 (2006.01); C12N 7/00 (2006.01); C12N 15/10 (2006.01); C12P 21/02 (2006.01); C07K 16/28 (2006.01); C07K 16/24 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); C07K 14/005 (2013.01); C07K 14/62 (2013.01); C07K 16/241 (2013.01); C12N 7/00 (2013.01); C12N 2740/16051 (2013.01); C12N 2740/16311 (2013.01);
Abstract

This application provides methods and compositions related to constructing nucleic acid hydrogels (e.g., RNA hydrogels) having repetitive monomer units, each monomer unit includes one or more G-quadruplex sequences. These G-quadruplex sequences cross-link the nucleic acid concatemer such that it self-assembles into a hydrogel under appropriate conditions. In some embodiments, each monomeric unit of the nucleic acid concatemer comprises a coding sequence for polypeptide of interest; and the nucleic acid hydrogel formed by the nucleic acid concatemer can be used for expressing the polypeptide in high quantities. In some embodiments, at least two RNA concatemers comprising G-quadruplex sequences are produced, one further comprising a spacer and the other further comprising a sequence encoding a polypeptide of interest. These two RNA concatemers are combined and self assembled to form a single, wideband RNA hydrogel.


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