The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 07, 2023

Filed:

Aug. 07, 2020
Applicant:

Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);

Inventors:

Gang Chen, Yorktown Heights, NY (US);

Robert Babb, River Edge, NJ (US);

James P. Fandl, LaGrangeville, NY (US);

Assignee:

Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/00 (2006.01); G01N 33/569 (2006.01); C07K 16/22 (2006.01); C07K 16/32 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
G01N 33/56972 (2013.01); C07K 16/00 (2013.01); C07K 16/22 (2013.01); C07K 16/32 (2013.01); G01N 33/50 (2013.01); C07K 2317/10 (2013.01); C07K 2317/92 (2013.01); C12N 2510/02 (2013.01);
Abstract

The present disclosure relates to a method for identifying cells that express antigen-specific antibodies with a high binding affinity for a monomeric antigen. Using fluorescence activated cell sorting, cells expressing high affinity antigen-specific antibodies are selected from a population of immune cells isolated from a mammal that has been immunized with or otherwise exposed to the antigen. Nucleic acids encoding the high affinity antibodies can then be cloned into other lymphoid and non-lymphoid cells where the antibody can be expressed and from which the antibodies can be secreted.


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