The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 07, 2023

Filed:

Aug. 13, 2018
Applicant:

The Governing Council of the University of Toronto, Toronto, CA;

Inventors:

Mahmoud Labib, Toronto, CA;

Edward H. Sargent, Toronto, CA;

Shana O. Kelley, Toronto, CA;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6816 (2018.01); C12Q 1/6886 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6816 (2013.01); C12Q 1/6886 (2013.01); C12Q 2563/143 (2013.01); C12Q 2563/155 (2013.01); C12Q 2565/629 (2013.01); C12Q 2600/158 (2013.01);
Abstract

Methods and systems for mRNA analysis and quantification of mRNA expression in cells are provided. An example method includes introducing a first capture probe and a second capture probe into the cells, the first capture probe and the second capture probe each configured to be complementary to a respective section of target mRNA within the cells, wherein binding of the first and second capture probes to the respective sections of the target mRNA results in tagging of the cells and causes the first and second capture probes to form clusters with each other. The first capture probe and the second capture probe are each bound to magnetic nanoparticles (MNPs) that, when trapped within the tagged cells, cause the tagged cells to be susceptible to magnetic forces. The method and system further include introducing the cells into a device configured to magnetically capture tagged cells.


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