The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 07, 2023
Filed:
Mar. 24, 2016
The Broad Institute, Inc., Cambridge, MA (US);
Dana-farber Cancer Institute, Inc., Boston, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
Asaf Rotem, Cambridge, MA (US);
Kevin Struhl, Cambridge, MA (US);
Paul Blainey, Cambridge, MA (US);
Liyi Xu, Cambridge, MA (US);
THE BROAD INSTITUTE, INC., Cambridge, MA (US);
DANA FARBER CANCER INSTITUTE, INC., Boston, MA (US);
PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US);
MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US);
Abstract
A method for detecting oncogenic growth and viability, and/or degree of cellular transformation and/or identifying an agent that inhibits cellular transformation is disclosed. The method including: providing a cellular sample, such as a sample of cells obtained from a subject or a cell line; culturing the cellular sample in low attachment conditions; and detecting growth and7or cell viability of the sample, wherein increased growth relative and/or viability relative to a control or control level indicative of basal growth and/or viability indicates cellular transformation. In some embodiments, the method includes introducing a n expression vector into cells of the cellular sample, wherein the expression vector comprises a gene product expression sequence being tested for transformation ability. In some embodiments the cellular sample is contacted with a test agent and growth and/or cell viability of the sample is determined to determine if the agent inhibits transformation.