The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 07, 2023

Filed:

Aug. 19, 2021
Applicants:

Kyoto University, Kyoto, JP;

Ushio Denki Kabushiki Kaisha, Toyota, JP;

Inventors:

Kenichiro Kamei, Kyoto, JP;

Yong Chen, Kyoto, JP;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/12 (2006.01); C12M 3/06 (2006.01); C12M 1/42 (2006.01); C12N 1/04 (2006.01); C12N 5/074 (2010.01); C12N 5/00 (2006.01); B33Y 80/00 (2015.01);
U.S. Cl.
CPC ...
C12M 25/14 (2013.01); C12M 23/16 (2013.01); C12M 35/08 (2013.01); C12N 1/04 (2013.01); C12N 5/0062 (2013.01); C12N 5/0696 (2013.01); B33Y 80/00 (2014.12); C12N 2500/50 (2013.01); C12N 2501/999 (2013.01); C12N 2513/00 (2013.01); C12N 2521/00 (2013.01); C12N 2523/00 (2013.01); C12N 2533/30 (2013.01); C12N 2539/10 (2013.01);
Abstract

The invention provides a microfluidic device comprising at least one cell culture chamber, the at least one cell culture chamber being connected to at least two openings, the device being configured to supply at least one physiologically active substance from at least one of the openings to the at least one cell culture chamber in such a manner as to form a concentration gradient or concentration gradients in the at least one chamber when cells and a hydrogel are introduced into the at least one cell culture chamber to culture the cells in a 3D-gel medium.


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