The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 31, 2023

Filed:

Nov. 18, 2019
Applicant:

The Governing Council of the University of Toronto, Toronto, CA;

Inventors:

Andrew Emili, Needham, MA (US);

Megan McLaughlin, Red Deer, CA;

Kyrylo Zagorovsky, Toronto, CA;

Jonathan Buchanan Olsen, Kitchener, CA;

Warren C. W. Chan, Toronto, CA;

Sachdev S. Sidhu, Toronto, CA;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 31/00 (2006.01); G01N 33/53 (2006.01); G01N 33/68 (2006.01); A61K 45/06 (2006.01); C07K 14/245 (2006.01); G01N 33/58 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6824 (2013.01); A61K 45/06 (2013.01); C07K 14/245 (2013.01); G01N 33/582 (2013.01); G01N 2333/952 (2013.01);
Abstract

The invention describes methods and reagents useful for sequencing polypeptide molecules. The method comprises affixing a polypeptide to a substrate and contacting the polypeptide with a plurality of probes. Each probe selectively binds to an N-terminal amino acid or an N-terminal amino acid derivative. Probes bound to the polypeptide molecule are then identified before cleaving the N-terminal amino acid or N-terminal amino acid derivative of the polypeptide. Also provided are methods for the sequencing a plurality of polypeptide molecules in a sample and probes specific for N-terminal amino acids or N-terminal amino acid derivatives.


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