The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 31, 2023

Filed:

Aug. 15, 2019
Applicants:

Massachusetts Institute of Technology, Cambridge, MA (US);

Beth Israel Deaconess Medical Center, Inc., Boston, MA (US);

Inventors:

Edward Stuart Boyden, Chestnut Hill, MA (US);

Andrew Hanno Beck, Brookline, MA (US);

Yongxin Zhao, Quincy, MA (US);

Octavian Bucur, Waltham, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/30 (2006.01); G01N 33/569 (2006.01); G01N 1/28 (2006.01);
U.S. Cl.
CPC ...
G01N 33/56966 (2013.01); G01N 1/28 (2013.01); G01N 1/30 (2013.01); G01N 2800/34 (2013.01);
Abstract

The invention provides a method for preparing an expanded renal (kidney) tissue sample suitable for microscopic analysis. Expanding the kidney sample can be achieved by binding, e.g., anchoring, key biomolecules to a polymer network and swelling, or expanding, the polymer network, thereby moving the biomolecules apart as further described herein. As the biomolecules are anchored to the polymer network, isotropic expansion of the polymer network retains the spatial orientation of the biomolecules resulting in an expanded, or enlarged, kidney sample.


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