The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 24, 2023

Filed:

Apr. 13, 2018
Applicant:

Juno Therapeutics, Inc., Seattle, WA (US);

Inventors:

Paul Ken Kodama, Seattle, WA (US);

Tom Kowski, Seattle, WA (US);

Mima Mujacic, Seattle, WA (US);

Kenneth Mayo Prentice, Seattle, WA (US);

Assignee:

Juno Therapeutics, Inc., Seattle, WA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); C12Q 1/34 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5308 (2013.01); C12Q 1/34 (2013.01); C12Y 305/01052 (2013.01); G01N 33/502 (2013.01); G01N 2400/10 (2013.01); G01N 2570/00 (2013.01);
Abstract

Provided herein are methods for assessing cell surface glycans, e.g., N-glycans, by assessing a sample of released surface glycans, and determining the presence, absence, or level of glycans present in the sample. Also provided are methods of assaying and/or evaluating a cell composition by assessing the cell surface glycan profile of the cell composition and comparing the profile to a reference sample. Methods for manufacturing and/or culturing a plurality of cell compositions having consistent surface glycan expression with low variability are also provided.


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