The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 24, 2023
Filed:
Mar. 16, 2022
Guangzhou Hybribio Medicine Technology Ltd., Guangdong, CN;
Guangdong Hybribio Biotech Co., Ltd., Guangdong, CN;
Yan Zheng, Guangdong, CN;
Jiayan Ma, Guangdong, CN;
Xiaowei Feng, Guangdong, CN;
Mengyu Liu, Guangdong, CN;
Peixuan Chen, Guangdong, CN;
Zhisheng Guan, Guangdong, CN;
Longxu Xie, Guangdong, CN;
Yiyuan Ge, Guangdong, CN;
GUANGZHOU HYBRIBIO MEDICINE TECHNOLOGY LTD., Guangdong, CN;
GUANGDONG HYBRIBIO BIOTECH CO., LTD., Guangdong, CN;
Abstract
The present invention discloses a method for isolating placental trophoblast cells from cervical exfoliated cells of a pregnant woman. Based on a specific antigen or combination expressed on the surface or inside of specific trophoblast cells, the designed microfluidic sorting chip or flow cytometer is used in the method to perform cell sorting of a cell suspension of a placental trophoblast sample, thus obtaining isolated and purified placental trophoblast cells. Compared with conventional methods, the method of the present invention has the advantages of non-invasively obtaining specimens and good specificity. Moreover, the method causes low risk of infection and abortion, allows earlier sampling time and can achieve the synchronous labeling of a plurality of antigens as well as identification and sorting of characteristic fluorescence signals; and the method has greatly improved accuracy and higher reliability and broader coverage area of detection results.