The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 24, 2023

Filed:

Jul. 15, 2019
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

George A. Fry, San Carlos, CA (US);

Christina E. Inman, San Mateo, CA (US);

John Bridgham, Hillsborough, CA (US);

Timothy Hunkapiller, Mercer Island, WA (US);

Charles S. Vann, El Granada, CA (US);

Assignee:

Life Technologies Corporation, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6869 (2018.01); G01N 35/10 (2006.01); B01L 3/00 (2006.01); G01N 35/00 (2006.01); G01N 21/11 (2006.01); B01F 33/30 (2022.01); B01F 33/40 (2022.01); B01J 19/00 (2006.01); C12Q 1/6874 (2018.01); G02B 21/34 (2006.01); G01N 21/05 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); B01F 33/30 (2022.01); B01F 33/403 (2022.01); B01J 19/0046 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); C12Q 1/6874 (2013.01); G01N 21/11 (2013.01); G01N 35/00069 (2013.01); G01N 35/0099 (2013.01); G01N 35/1002 (2013.01); B01J 19/0093 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/00351 (2013.01); B01J 2219/00466 (2013.01); B01J 2219/00511 (2013.01); B01J 2219/00527 (2013.01); B01J 2219/00576 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00648 (2013.01); B01J 2219/00659 (2013.01); B01J 2219/00691 (2013.01); B01J 2219/00722 (2013.01); B01J 2219/00869 (2013.01); B01L 3/5025 (2013.01); B01L 3/502707 (2013.01); B01L 2200/025 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0822 (2013.01); B01L 2300/0851 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/1822 (2013.01); B01L 2400/0427 (2013.01); G01N 21/05 (2013.01); G01N 35/1011 (2013.01); G01N 35/1097 (2013.01); G01N 2035/00148 (2013.01); G01N 2035/00237 (2013.01); G01N 2035/1034 (2013.01); G02B 21/34 (2013.01); Y10T 436/11 (2015.01); Y10T 436/143333 (2015.01);
Abstract

According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.


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