The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 24, 2023
Filed:
Apr. 05, 2021
Applicant:
The Regents of the University of Colorado, Denver, CO (US);
Inventors:
Andrew Garst, Boulder, CO (US);
Ryan T Gill, Denver, CO (US);
Assignee:
THE REGENTS OF THE UNIVERSITY OF COLORADO, Denver, CO (US);
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/90 (2006.01); C12N 15/10 (2006.01); C40B 40/08 (2006.01); C12N 15/11 (2006.01); C12N 9/22 (2006.01);
U.S. Cl.
CPC ...
C12N 15/902 (2013.01); C12N 9/22 (2013.01); C12N 15/102 (2013.01); C12N 15/1024 (2013.01); C12N 15/1082 (2013.01); C12N 15/1093 (2013.01); C12N 15/11 (2013.01); C12N 15/111 (2013.01); C40B 40/08 (2013.01); C12N 2310/10 (2013.01); C12N 2310/20 (2017.05); C12N 2320/11 (2013.01); C12N 2320/12 (2013.01); C12N 2330/31 (2013.01);
Abstract
Described herein are method for generating a vector for editing a cell. The method comprises ligating into a vector that encodes a portion of a gRNA a cassette comprising at least one editing cassette, a promoter, and a gene encoding another portion of the gRNA. Upon ligation, the portion of the gRNA from the editing cassette and the other portion of the gRNA are ligated and form a functional gRNA.