The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 17, 2023

Filed:

Apr. 23, 2020
Applicant:

Hitachi High-tech Corporation, Tokyo, JP;

Inventors:

Ryosuke Takahashi, Tokyo, JP;

Katsuhiro Kanda, Tokyo, JP;

Yusuke Shimizu, Tokyo, JP;

Hisashi Ichikawa, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/50 (2006.01); G01N 35/10 (2006.01); G01N 33/15 (2006.01); G01N 35/00 (2006.01); G01N 35/04 (2006.01); B01L 7/00 (2006.01); C12M 1/12 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); B01L 3/00 (2006.01); B01L 9/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5067 (2013.01); B01L 7/00 (2013.01); C12M 25/00 (2013.01); C12M 29/00 (2013.01); C12M 41/46 (2013.01); C12M 47/20 (2013.01); G01N 33/15 (2013.01); G01N 33/502 (2013.01); G01N 35/1009 (2013.01); B01L 3/5085 (2013.01); B01L 9/543 (2013.01); G01N 2035/00425 (2013.01); G01N 2035/0418 (2013.01);
Abstract

Application of the present invention enables quantification of fractions of candidate pharmaceutical compounds (a parent compound and its metabolites), one excreted to the basolateral (Basal/Basolateral)-side via transporters and by diffusion, one excreted to the lumen (Apical)-side, and one remained in the cells. This enables determination of the total amount of the administered candidate pharmaceutical compounds and the distribution ratio of the fractions. The kinetics of the administered candidate pharmaceutical compounds can be evaluated, thereby enabling in vitro screening of an enormous number of candidate pharmaceutical compounds for drug candidates exhibiting the efficacy. The object of the present invention is to provide an apparatus and method for understanding a total picture of pharmacokinetics in vitro by quantifying a fraction of basolateral (Basal/Basolateral) efflux, a fraction of lumen (Apical)-side excretion, and a fraction remaining in a cell of a drug which has been administered to the cell to determine the distribution ratio of each fraction.


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