The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 17, 2023

Filed:

Jun. 23, 2021
Applicant:

Roche Diagnostics Operations, Inc., Indianapolis, IN (US);

Inventors:

Harald Sobek, Biberach, DE;

Michael Greif, Penzberg, DE;

Marco Thomann, Penzberg, DE;

Sebastian Malik, Antdorf, DE;

Assignee:

Roche Diagnostics Operations, Inc., Indianapolis, IN (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/00 (2006.01); C12N 9/10 (2006.01); C12P 19/44 (2006.01); C07K 16/00 (2006.01);
U.S. Cl.
CPC ...
C12P 21/005 (2013.01); C07K 16/00 (2013.01); C12N 9/1081 (2013.01); C12P 19/44 (2013.01); C12Y 204/99001 (2013.01); C12Y 301/03 (2013.01); C07K 2317/41 (2013.01);
Abstract

The properties of certain glycosyltransferase variants having N-terminal truncation deletions or internal deletions are disclosed. Particularly, mutants that exhibit α-2,6-sialyltransferase enzymatic activity in the presence of CMP-activated sialic acid as co-substrate, and in the presence of a suitable acceptor site, are disclosed. A fundamental finding documented in the present disclosure is that enzymes are not only capable of catalyzing transfer of a sialidyl moiety but they are also capable of catalyzing hydrolytic cleavage of terminally bound sialic acid from a glycan.


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