IDiyas

For the Inventor, By the Inventor

The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11786872 B1

PDFFull Text
Date of Patent:
Oct. 17, 2023

Filed:

May. 01, 2020

Vitro evolution in microfluidic systems

Applicants:

President and Fellows of Harvard College, Cambridge, MA (US);

United Kingdom Research and Innovation, Swindon, GB;

Inventors:

Andrew David Griffiths, Strasbourg, FR;

David A. Weitz, Cambridge, MA (US);

Darren Roy Link, Lexington, MA (US);

Keunho Ahn, Boston, MA (US);

Jerome Bibette, Paris, FR;

Assignees:

UNITED KINGDOM RESEARCH AND INNOVATION, Swindon, GB;

PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US);

Attorneys:

Withers Bergman LLP

Thomas C. Meyers

Primary Examiner:

Narayan K Bhat

Int. Cl.
CPC ...
B01F 23/41 (2022.01); B01L 3/00 (2006.01); C12N 15/10 (2006.01); G01N 15/14 (2006.01); B01F 25/433 (2022.01); B01F 33/3011 (2022.01); B01F 33/302 (2022.01); B01F 33/3031 (2022.01); C12P 21/00 (2006.01); B01J 19/00 (2006.01); C12Q 1/6874 (2018.01);
U.S. Cl.
CPC ...
B01F 23/41 (2022.01); B01F 25/433 (2022.01); B01F 25/4331 (2022.01); B01F 25/4338 (2022.01); B01F 33/3011 (2022.01); B01F 33/3021 (2022.01); B01F 33/3031 (2022.01); B01J 19/0046 (2013.01); B01L 3/502746 (2013.01); B01L 3/502753 (2013.01); B01L 3/502776 (2013.01); B01L 3/502784 (2013.01); C12N 15/1058 (2013.01); C12N 15/1075 (2013.01); C12P 21/00 (2013.01); C12Q 1/6874 (2013.01); G01N 15/1459 (2013.01); G01N 15/1484 (2013.01); B01J 2219/005 (2013.01); B01J 2219/0052 (2013.01); B01J 2219/00466 (2013.01); B01J 2219/00468 (2013.01); B01J 2219/00545 (2013.01); B01J 2219/00576 (2013.01); B01J 2219/00657 (2013.01); B01J 2219/00722 (2013.01); B01L 2200/0636 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0487 (2013.01); G01N 2015/149 (2013.01);
Abstract

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.

Find Patent Forward Citations

Loading…