The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 17, 2023
Filed:
May. 03, 2018
Applicant:
Seoul National University Hospital, Seoul, KR;
Inventors:
Eun Ju Lee, Seoul, KR;
Hyo-Soo Kim, Seoul, KR;
Assignee:
SEOUL NATIONAL UNIVERSITY HOSPITAL, Seoul, KR;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 15/113 (2010.01); C12N 15/85 (2006.01); C12N 15/09 (2006.01); A01K 67/027 (2006.01); C07K 14/72 (2006.01); C12N 9/22 (2006.01);
U.S. Cl.
CPC ...
A01K 67/0275 (2013.01); C07K 14/72 (2013.01); C12N 9/22 (2013.01); C12N 15/113 (2013.01); C12N 15/8509 (2013.01); A01K 2217/07 (2013.01); A01K 2227/105 (2013.01); C07K 2319/09 (2013.01);
Abstract
Provided are a protein complex in which estrogen receptor 2 (-ERT2) is fused to CRISPR associated protein 9 (Cas9), and a recombinant vector carrying a gene coding the protein complex, wherein ERT2 is bonded to the N-terminus and C-terminus of nuclear localization sequence (NLS)-removed Cas9 and the complex has the advantage of translocating from the cytosol into the nucleus at a certain time point upon treatment with tamoxifen and modifying a specific DNA with the aid of guide RNA (gRNA), ultimately enabling a more elaborate DNA modification operation in a desired part at a desired time point.