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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11783916 B1

PDFFull Text
Date of Patent:
Oct. 10, 2023

Filed:

Dec. 16, 2020

System and method for acquisition and processing of multiplexed fluorescence in-situ hybridization images

Applicant:

Applied Materials, Inc., Santa Clara, CA (US);

Inventors:

Yun-Ching Chang, San Jose, CA (US);

Dan Xie, Dublin, CA (US);

Chloe Kim, San Jose, CA (US);

Assignee:

Applied Materials, Inc., Santa Clara, CA (US);

Attorney:

Fish & Richardson P.C.

Primary Examiner:

Shervin K Nakhjavan

Int. Cl.
CPC ...
G06K 9/00 (2022.01); G16B 40/10 (2019.01); G02B 21/00 (2006.01); G01N 21/64 (2006.01); C12Q 1/6841 (2018.01); G02B 21/16 (2006.01); G02B 21/36 (2006.01); G06T 5/00 (2006.01); G06T 5/20 (2006.01); G06T 5/50 (2006.01); G06T 7/00 (2017.01); G16B 50/40 (2019.01); G06V 10/30 (2022.01); G06V 20/69 (2022.01); G06V 10/50 (2022.01); G06V 10/75 (2022.01); G16B 50/00 (2019.01); G01N 21/75 (2006.01);
U.S. Cl.
CPC ...
G16B 40/10 (2019.02); C12Q 1/6841 (2013.01); G01N 21/6428 (2013.01); G01N 21/6456 (2013.01); G01N 21/6458 (2013.01); G02B 21/0076 (2013.01); G02B 21/16 (2013.01); G02B 21/365 (2013.01); G02B 21/367 (2013.01); G06T 5/003 (2013.01); G06T 5/006 (2013.01); G06T 5/20 (2013.01); G06T 5/50 (2013.01); G06T 7/0012 (2013.01); G06V 10/30 (2022.01); G06V 10/507 (2022.01); G06V 10/758 (2022.01); G06V 20/69 (2022.01); G06V 20/698 (2022.01); G16B 50/00 (2019.02); G16B 50/40 (2019.02); G01N 2021/6419 (2013.01); G01N 2021/6421 (2013.01); G01N 2021/6441 (2013.01); G01N 2021/755 (2013.01); G01N 2201/12761 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/30004 (2013.01); G06T 2207/30168 (2013.01);
Abstract

In acquisition of spatial transcriptomic information, a plurality of images representing a common field of view of a sample are obtained and registered. Each pixel of the registered images is decoded by identifying a code word from a plurality of code words in a code book that provides a best match to data values in the plurality of registered images for the pixel. For each code word identified as a best match and each pixel, whether a bit ratio for an image word for the pixel meets a threshold for the code word is determined. The image word is formed from the data values in the plurality of registered images for the pixel. For at least one pixel that is determined to meet the threshold, a gene associated with the code word is determined. Pixels for which the bit ratio does not meet the threshold are screened.

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