The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 10, 2023
Filed:
Apr. 25, 2017
Applicant:
Trizell Ltd., Chinnor, GB;
Inventors:
Hanna P. Lesch, Kuopio, FI;
Joonas Malinen, Kuopio, FI;
Eevi Lipponen, Kuopio, FI;
Anniina Valkama, Kuopio, FI;
Hanna Leinonen, Kuopio, FI;
Assignee:
Trizell Ltd., West Drayton, GB;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); C12M 1/02 (2006.01); C12P 21/02 (2006.01); C12M 1/12 (2006.01); C12N 15/86 (2006.01); C12M 1/34 (2006.01); C07K 14/005 (2006.01);
U.S. Cl.
CPC ...
C12M 29/18 (2013.01); C07K 14/005 (2013.01); C12M 1/02 (2013.01); C12M 25/18 (2013.01); C12M 29/10 (2013.01); C12M 41/26 (2013.01); C12M 41/46 (2013.01); C12N 15/86 (2013.01); C12P 21/02 (2013.01); C12N 2740/15051 (2013.01);
Abstract
We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and COare managed during transfection.