The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 10, 2023
Filed:
Oct. 30, 2020
Applicant:
Takeda Pharmaceutical Company Limited, Osaka, JP;
Inventors:
Assignee:
Takeda Pharmaceutical Company Limited, Osaka, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/755 (2006.01); C12N 9/64 (2006.01); A61K 9/00 (2006.01); A61K 38/36 (2006.01); A61K 38/37 (2006.01); C12P 21/00 (2006.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
C07K 14/755 (2013.01); A61K 9/0019 (2013.01); A61K 38/36 (2013.01); A61K 38/37 (2013.01); C12N 9/6489 (2013.01); C12P 21/00 (2013.01); A61K 38/00 (2013.01); C12N 2500/24 (2013.01); C12N 2511/00 (2013.01); C12Y 304/24087 (2013.01);
Abstract
Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.