The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 03, 2023

Filed:

May. 27, 2020
Applicant:

Wuxi Biologics Ireland Limited, Dundalk, IE;

Inventors:

Jun Hu, Shanghai, CN;

Gong Chen, Shanghai, CN;

Yongjun Qin, Shanghai, CN;

Weichang Zhou, Shanghai, CN;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01J 3/44 (2006.01); C12M 1/00 (2006.01); G01N 21/65 (2006.01);
U.S. Cl.
CPC ...
G01J 3/44 (2013.01); C12M 21/00 (2013.01); C12M 29/10 (2013.01); G01N 21/65 (2013.01);
Abstract

A Raman spectroscopy integrated perfusion cell culture system for monitoring and auto-controlling perfusion cell culture, comprising: a bioreactor, comprising: (1) an interior chamber for receiving a cell culture, (2) a port for feeding nutrient materials from a feed reservoir into the interior chamber, (3) a port for auto-bleeding from the interior chamber, and (4) a port for continuously harvesting materials from the interior chamber with the help of a cell retention system; a Raman analyzer, comprising (i) one or more Raman probes which are immersed into the bioreactor and is configured to collect Raman spectrum in the bioreactor, and (ii) a host computer which is configured to receive the Raman spectrum collected and transferred by the Raman probe and turn it into values; and a controller in communication with the Raman analyzer, the feed reservoir and an auto-bleeding device, the controller being configured to receive the values from the Raman analyzer and compare them with preset parameters, based on results from comparing, the controller further being configured to control the feed reservoir for adjusting the feeding rate of nutrient materials into the bioreactor, and being configured to control the auto-bleeding device for auto-bleeding materials from the bioreactor. A process for monitoring and auto-controlling perfusion cell culture by using this system.


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