The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 03, 2023

Filed:

Jan. 16, 2019
Applicant:

Ningbo Health Gene Technologies Co., Ltd., Zhejiang, CN;

Inventors:

Ding Yu, Zhejiang, CN;

Yong Wu, Zhejiang, CN;

Haiying Jin, Zhejiang, CN;

Kang Wang, Zhejiang, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6888 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6888 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/16 (2013.01);
Abstract

Provided is a multiplex PCR amplification method for identifying an unknown biological sample suspected to be from a human. The method comprises the following steps: 1) acquiring an unknown biological sample suspected to be from a human and which is to be detected; 2) directly adding said unknown sample or nucleic acid extracted from said unknown sample to a premixed PCR reagent; 3) running a PCR amplification program to conduct a multiplex PCR amplification reaction; and 4) detecting and analyzing PCR amplification products, wherein the premixed PCR reagent contains primers specific to human genetic markers and primers specific to nuclear chromosomal genes of non-human species. Also provided is a multiplex PCR amplification kit for species and human individual recognition and identification of an unknown biological sample.


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